November 2, 2007
The following are Microscopy websites which Alison North recommends:
The Molecular Expressions Microscopy Primer - Introduction to Microscopy
The Olympus Microscopy Resource Center
Fluorescent Tutorials - Invitrogen - Molecular Probes
We will add other sites as suggested by other faculty and attendees.
November 2, 2007
All the faculty of Principles and Applications of Immunocytochemistry have published articles which focus on their particular presentations. Below you will find links to articles which faculty members have chosen as representative of their work.
Richard W. Burry
Richard W. Burry Specificity Controls for Immunocytochemical Methods J. Histochem. Cytochem., 48: 163-166, 2000. -
Richard W. Burry and Catherine L. Smith
HuD Distribution changes in Response to Heat Shock but Not Neurotrophic Stimulation
J. Histochem. Cytochem. 54:1129-1138, 2006.
Kevin A. Roth
Shindler KS, Roth KA: Double immunofluorescent staining using two unconjugated primary antisera raised in the same species. J. Histochem. Cytochem. 1996; 44:1331-1335. PubMed ID: 8918908
Zaidi AU, Enomoto H, Milbrandt J, Roth KA: Dual fluorescent in situ hybridization and immunohistochemistry with tyramide signal amplification. J. Histochem. Cytochem. 2000; 48:1369-1376. PubMed ID: 10990490
Ness JM, Akhtar RS, Latham CB, Roth KA: Combined tyramide signal amplification and quantum dots for sensitive and photostable immunofluorescence. J. Histochem. Cytochem., 2003; 51:981-987. PubMed ID: 12871979
Roth KA: A Beautiful Science. J. Histochem. Cytochem. 2006; 54:1073-1074. PubMed ID: 16980508
Alison G. North
Alison J. North. Seeing is believing? A beginners’ guide to practical pitfalls in image acquision.
J. Cell Biol. 2006 172: 9-18, 2006, 10.1083/jcb.200507103.
Helen Pearson The good, the bad and the ugly. Nature 447, 138-140, 2007. Alison is quoted extensively in this article about the difference between a pretty image and a good image.
October 26, 2007
Bill Stahl on Immunocytochemistry
I have been using immunocytochemical techniques in my research for over thirty years.
I sometimes get asked what is immunocytochemistry and what do you do with it? Immunocytochemistry is the process of localizing antigens in biological tissues and cells using target specific antibodies. More formally immunohistochemistry refers to the process as applied to tissues and immunocytochemistry refers to localization in cells but the terms are often used interchangeably. The most typical sites identified by immunocytochemistry are proteins, but lipids and other cellular constituents can also be localized both by light, fluorescence and electron microscopy. Applications extend from research in cell biology to localization of specific markers in specimens sent to the pathology lab to support clinical diagnoses
Almost all biologists utilize ICC in their work.
It’s an extremely useful scientific method for research but it can be misunderstood in its implementation and interpretation. It’s easy to purchase a commercial kit to carry out an immunocytochemistry application but a basic understanding of underlying principles and potential problems is essential if you want to be successful in your use of ICC. It also helps to get your papers published and your research grants funded.
Subscribe to this feed.
