PRINCIPLES AND APPLICATIONS OF IMMUNOCYTOCHEMISTRY SYLLABUS
8:00 AM 1. Introduction - (10 min)
William Stahl
The Histochemical Society and Univ. Washington School of Medicine, Seattle
8:15 AM 2 Concepts related to the practice of immunocytochemistry
Gwen V. Childs (40 min + 5 min discussion)
Univ. Arkansas School of Medicine, Little Rock
9:00 AM 3. Sample preparation – fixation– (30 + 5 min discussion)
Richard Burry
Ohio State University, Columbus, OH
9:35 AM 4. Antibodies – (30 + 5 min discussion)
Allen Gown
PhenoPath Laboratories, Seattle, WA
10:10 AM Coffee Break
10:25 AM 5. Detection methods: The Labels, Direct, Indirect, ABC; Label types - fluorescence, HRP, amplification, Q-dots, etc. (40 min +5 min discussion)
Richard Burry
Ohio State University, Columbus, OH
11:10 AM 6. Detection methods: The Procedures, Tissue prep, reagents, antibodies, protocols, microscopy, etc. 30 min +5 min discussion)
Richard Burry
Ohio State University, Columbus, OH
11:45 AM 7. Antigen retrieval (30 min +5 min discussion)
Allen Gown
PhenoPath Laboratories, Seattle, WA
12:20 AM Lunch
1:00 PM 8. Amplification methods (30 min +5 min discussion))
Kevin Roth
Univ. Alabama School of Medicine, Birmingham) and the Journal of Histochemistry and Cytochemistry.
1:35 PM 9. Double-labeling and localization strategies. (45 min +5 min discussion)
Gloria Hoffman
University of Maryland at Baltimore
2:25 PM 10. Controls (30 + 5 min discussion)
Richard Burry
Ohio State University, Columbus, OH
3:00 PM 11. Interpreting microscopic images (40 min + 5 min discussion)
Alison J. North
Rockefeller University, New York, NY
3:45PM 12. Closing remarks and questions from the audience
Denis G. Baskin
University of Washington School of Medicine and VA Puget Sound Medical Center and the Journal of Histochemistry and Cytochemistry
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